Identification of functional cytochrome P450 and ferredoxin from Streptomyces sp. EAS-AB2608 by transcriptional analysis and their heterologous expression

Bioconversion using microorganisms and their enzymes is an important tool in many industrial fields. The discovery of useful new microbial contributes to the development industries utilizing bioprocesses. Streptomyces sp. EAS-AB2608, isolated from a soil sample collected Japan, can convert tetrahydrobenzotriazole CPD-1 (a selective positive allosteric modulator metabotropic glutamate receptor 5) its hydroxylated form at C4-(R) position. current study was performed identify genes encoding involved bioconversion verify function. To gene products responsible for conversion CPD-1, we used RNA sequencing analyze EAS-AB2608; 8333 coding sequences, selected two genes, one cytochrome P450 (easab2608_00800) other ferredoxin (easab2608_00799), as desirable CPD-1. validity this selection tested by heterologous expression approach. A assay genetically engineered avermitilis SUKA24 ?saverm3882 ?saverm7246 co-expressing (strain ES_SUKA_63) confirmed that these had hydroxylation activity with respect indicating they are Strain ES_SUKA_63 also showed oxidative toward compounds therefore might be not only but synthesis drug metabolites optimization studies various pharmaceutical lead compounds. We expect approach will bridging gap between latest enzyme technologies conventional screening microorganisms. • Genes easab2608_00800 (cyp) easab2608_00799 (fdx) were RNA-Seq. Selection evaluated S. system.